Coding
INT10

Part:BBa_K3254009:Design

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-14)


Integrase Int10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original sequence was first reported in 2014. In this version，two PstI sites were eleminated by synonymous mutations.


Source

The original organism is the Streptococcus pneumoniae 670-6B. The DNA of this part was coloned from the source plasmid(pCis7+10+8, see reference).

References

Yang, L., et al., Permanent genetic memory with >1-byte capacity. Nat Methods, 2014. 11(12): p. 1261-6.